Bd facscanto ii flow cytometer quick reference guide. Intended use the bd facscanto system is intended for use as an in vitro diagnostic device for identification and enumeration of lymphocyte subsets in human cells in suspension for flow cytometry. Bd facscanto ii flow cytometer quick reference guide this guide contains instructions for both bd facscanto clinical and bd facsdiva software. Turn on the canto ii at the power button red circle and right in detail. Wellington, auckland, new zealand ec rep bd facsdiva software reference manual for version 8. Make sure the following steps are done before running your samples. New look and feel the bd facsdiva software interface has been updated to provide a more intuitive look and feel. For applicatio nspecific instructions, refer to the individual. The bd facscanto ii system brings bd flow cytometry and multicolor analysis to a new level to help researchers meet complex challenges and advance their research. One striking difference between the facscanto and older generation machines is that this system operates on a. Sections are indicated with the appropriate software icons.
Built on more than 25 years of bd experience and leadership in flow cytometry and multicolor analysis, the. Wait 10 seconds, then open the bd facsdiva software and log on. Bd facscanto ii flow cytometer reference manual free ebook download as pdf file. Pdf files can also be created for carousel run reports and batch analysis reports. Make sure that the facsflow pbs level is high and that the waste is low 5. How to operate the bd facscanto flow cytometer preface dear colleague, the bd facscanto flow cytometer is use to operate, however, it is a delicate instrument for measuring fluorescence of single cells. Todays bd facscanto system features a fixedalignment flow cell in the fluidics system that minimizes startup time and improves reproducibility. Bd facscanto ii bd pdf catalogs technical documentation. Bd facscanto ii flow cytometer technical specifications.
The bd facsaria iii analysis performance is comparable to stateoftheart highly sensitive analyzers. Bd facsdiva software quick reference guide for the bd lsr ii. When you install tubes onto the sample injection tube sit, a pump. Bd facscanto ii flow cytometer, and includes features for platebased acquisition using the bd high throughput sampler hts on both the bd facscanto and bd facscanto ii flow cytometer. This guide should help you operating the flow cytometer and leaving it such way that another colleague can use it another day. Becton dickinson pty ltd, 4 research park drive, macquarie university research park, north ryde nsw 21, australia becton dickinson limited, 8 pacific rise, mt. System options include an automated sample loader and a barcode reader. Bd facscanto ii flow cytometers were used in six laboratories and bc navios flow cytometers in four laboratories. Facscanto ii standard operation protocol basic operation. The bd facscanto ii system features many innovations. Check that the sheath cube is full and the waste tank is empty.
Page 1 bd facscanto ii instructions for use for in vitro diagnostic use part no. When you are finished and if you are not the last booked user for the day, log out of your centre folder by selecting file log. At the heart of the cytometer, for example, the fluidics system features a fixedalignment flow cell to minimize startup time and improve. In the fluidics system, the sample travels up the sample injection tube, and hydrodynamic focusing within the flow cell forces particles into a single file.
Bd facsdiva software contains vxworks as embedded software runtime module. Bd facscanto ii flow cytometry system becton dickinson. Follow only those sections that apply to the software you are running. Bd biosciences is not liable for any claims related to or resulting from buyerusers failure to install and maintain virus protection. Since then, there have been many innovations in the product line, reflecting our commitment to reach to ever higher standards.
If you are familiar with a previous version of bd facsdiva software, you will notice that many of its icons have. System components the bd facscanto system consists of a benchtop flow cytometer, a self contained fluidics cart, and the bd facscanto workstation. Bd facscanto ii instructions for use manual pdf download. The solution helps reduce errors due to manual transcriptions. The xml experiment file can only be opened in facs diva but the fcs files can be used in flowjo software for further analysis. Click no to overwrite your previous file with the new file. Bd facscanto clinical software c instrument qc, lymphocyte subset tbnk, hlab27, stem cell enumeration bd facsdiva. Reading this material and answering the questions at the end of each section will enhance your handson training experience during operator training at bd biosciences. At the heart of the cytometer, for example, the fluidics system features a fixedalignment flow cell to minimize startup time and improve reproducibility. Aug 14, 2019 bd facsdiva software quick reference guide for the bd lsr ii. This design architecture achieves high numerical aperture light collection. The bd facscanto family of benchtop analyzers features reliable performance and accurate results for experiments requiring up to 10 parameters.
Standardization of 8color flow cytometry across different. Facscanto flow cytometer from bd biocompare product. Saving worksheets as pdf files on page 194 in the bd facsdiva software reference manual. Worksheet toolbar button to save the worksheet as a pdf file. Lysed whole human blood eightcolor immunophenotyping panel using fitc. Even when acquiring samples using the hts, consult the appropriate. Electronics to convert the optical signals to proportional digital. The bd facscanto ii system brings bd flow cytometry and multicolor analysis to a new level to help researchers meet complex challenges.
Bd facs canto ii flow cytometer quick user guide v. Bd facscanto ii operation manual 1 facscanto ii standard operation protocol basic operation 1. There is enough amount of sheath fluid to perform machine start up and sample acquisition. The bd facscanto ii provides the ultimate in flexibility and can be configured with two or three lasers to detect up to eight colors. Carousel report now includes the directory selected for each specimens data in the carousel. Export selected worksheet elements as an xml file including plots, text. The bd facscanto is often hailed as the new generation of flow cytometry. Select file exit to close the cst window and connect back to the bd facsdiva interface. Default templates are now provided for certain instrument functions. Download user manual manual of bd facscanto ii laboratory equipment, measuring instruments for free or view it online on. It is the responsibility of the buyeruser to ensure that all added electronic files including software and transport media are virus free. I imported bd facscanto clinical software files into bd facsdiva software. Bd biosciences bd facscanto ii flow cytometers 422 and 53 configurations with bd facscanto clinical software and bd facsdiva software.
Click cancel to return to the results preferences window. All 3laser instruments were equipped with their standard optical configuration for a blue laser emitting at 488 nm, a red laser emitting at 633 nm bd facscanto ii or 638 nm bc navios, and a violet laser emitting at 405 nm. Bd biosciences technical resources page 5 august 2012 bd facscanto ii flow cytometer and bd facscanto clinical software faqs click yes to back up your existing file. Launch tera term, click on serial and make sure the port is on com1 and click ok 4. The spa iii automates a range of manual sample preparation steps.
Before you begin this guide assumes that you have read the bd. Bd facscanto ii measuring instruments user manual manual pdf. Flow cytometer on page 16 fluidics cart on page 26 computer workstation on page 29. Bd facs canto ii operators manual marshall scientific issuu. If any parameters did not pass, refer to the cytometry supervisor or the bd cytometer setup and tracking application guide for help troubleshooting. This is accomplished by using a similar gelcoupled flow cell design and the fixed optical architecture. Bd facscantotm with hts users guide for authorization to use the facscanto ii cytometer, you first have to receive a training and must accept the user rules of the facility. Further details can be found in the reference manual, provided on the documentation cd, for either the bd facscanto or the bd facscanto ii flow cytometer. Numerous innovations make the system easy to use, powerful, and productive.
One striking difference between the facscanto and older generation machines is that this system operates on a pcbased windows xp platform using bd facsdiva software. Further details can be found in the reference manual, provided on the documentation cd, for either the bd facscanto. Bd biosciences does not make any warranty with respect to the workstation remaining virus free after installation. The bd facscanto ii instructions for use assumes you have a working knowledge of basic microsoft windows operation. Even when acquiring samples using the hts, consult the appropriate cytometer users guide for information about flow cytometer operation, daily shutdown, maintenance, and troubleshooting. Bd facscanto ii measuring instruments user manual manual. Blue laser 488nm red laser 635nm violet laser 405nm. These tools assist in the ease and efficiency of using facscanto as they enable automated features. The first bd facscanto system was introduced in 2004. The bd facscanto ii system consists of a benchtop flow cytometer, a selfcontained fluidics cart, and the bd facscanto ii workstation.
New features available to all instruments include the following. We would like to show you a description here but the site wont allow us. In the fluidics system, the sample travels up the sample injection tube, and hydrodynamic focusing within the flow cell forces particles into a single file stream where laser light intercepts the stream at the sample interrogation point. Bd facscanto ii becton dickinson, san jose, ca, usa data file structure.
Compensation controls in the bd facsdiva software reference manual. This is accomplished by using a similar gelcoupled flow cell design and the fixed optical architecture of the bd facscanto ii and bd lsrfortessa systems. All 3laser instruments were equipped with their standard optical configuration for a blue. Intended use the bd facscanto system is intended for use as an in vitro diagnostic device for identification and enumeration of lymphocyte subsets in human cells in suspension for flow. See saving worksheets as pdf files on page 194 in the bd facsdiva software reference manual.
A fixed alignment flow cell, for example, minimizes startup time and improves reproducibility. Waste tank is empty and refill the bottom layer of waste tank with bleach. A june 2007 bd biosciences benex limited canada japan bay k 1 ad nippon becton. If a worksheet with the name of siftware specimen and tube already exists, the copied elements are pasted into the existing worksheet. A pdf file showing the number of cells that were sorted and which gate was used to sort.
How to operate the bd facscanto flow cytometer preface dear colleague, the bd facscanto flow cytometer is use to operate, however, it is a delicate instrument for measuring fluorescence of single. Do not attempt to read fcs files created with version 2. The bd facs canto ii offers three lasers and is able to detect up to 8 parameters fluorochromes at the same time. Unlock the screen with your ppms account utsw username and password 3. For the analysis at the canto ii you have the choice between two. Bd facscanto ii becton dickinson, san jose, ca, usa. At the heart of the cytometer, for example, the fluidics system features a fixed alignment flow cell to. Before you begin this guide assumes that you have read the bd facscanto ii instructions for use and the bd facscanto clinical software reference manual, and that you are familiar with cytometer setup. Bd facscanto ii flow cytometer reference manual alfresco. A june 2007 bd biosciences benex limited canada japan bay k 1 ad nippon becton dickinson 2350 qume drive toll free 888 2590187 company, ltd. Jun 07, 2006 the bd facscanto is often hailed as the new generation of flow cytometry. System options include an automated sample loader and a barcode. The bd facscanto ii fluidics system is designed to streamline work, save time, and improve performance. Bd biosciences is not liable for any claims related to or resulting from buyerusers failure.
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